This ready-to-use bacterial freezing medium falls under the category of Cell Freezing Reagents, tailored for cryopreservation of all common laboratory bacterial strains. It is sorted together with various Cell Culture Reagents in laboratory reagent inventory management, while differentiated from functional products like Cell Transfection Reagents used for gene delivery experiments.
Storage Conditions
The medium can be stored for 12 months at -20°C; long-term stable preservation is achievable at -70°C or -80°C. Subjected to high-pressure sterilization in advance, this bacterial cryopreservation medium can be applied directly. There is no need to collect bacterial precipitates; users only need to add the medium to bacterial liquid and blend evenly.
Standard Operating Protocol (for reference)
Culture bacteria until they reach logarithmic growth phase.
Prepare sterile cryotubes or centrifuge tubes, mix bacterial suspension and ready-to-use bacterial freezing medium at a volume ratio o…